A student uses restriction enzymes to cut a DNA molecule into fragments. The digested DNA is loaded into the wells of an agarose gel and the gel is subjected to an electric current. Upon completion of the run, the gel is stained.
(A) It increases the contrast between the agar and the DNA fragments.
(B) It must be accounted for when calculating the molecular weight of the fragments.
(C) Its charged areas interfere with the migration of the DNA.
(D) It is bonded only to the sticky ends of the fragments and can directly determine the sequence of the DNA fragments.
(E) It gives a three-dimensional view of the structure of the DNA fragments.(A) they influence the rate of migration of the fragmentsA student uses restriction enzymes to cut a DNA molecule into fragments. The digested DNA is loaded into the wells of an agarose gel and the gel is subjected to an electric current. Upon completion of the run, the gel is stained.
Answer: A
Which of the following is true of the dye used to stain the fragments
(A) It increases the contrast between the agar and the DNA fragments.
(B) It must be accounted for when calculating the molecular weight of the fragments.
(C) Its charged areas interfere with the migration of the DNA.
(D) It is bonded only to the sticky ends of the fragments and can directly determine the sequence of the DNA fragments.
(E) It gives a three-dimensional view of the structure of the DNA fragments.(A) they influence the rate of migration of the fragmentsA student uses restriction enzymes to cut a DNA molecule into fragments. The digested DNA is loaded into the wells of an agarose gel and the gel is subjected to an electric current. Upon completion of the run, the gel is stained.
Answer: A