In recombinant DNA methods, the term vector can refer to

In recombinant DNA methods, the term vector can refer to 

A) the enzyme that cuts DNA into restriction fragments.
B) the sticky end of a DNA fragment.
C) a RFLP marker.
D) a plasmid used to transfer DNA into a living cell.
E) a DNA probe used to identify a particular gene.

Answer: D

Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme?

Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme? 

A)
AAGG
TTCC
B)
AGTC
TCAG
C)
GGCC
CCGG
D)
ACCA
TGGT
E)
AAAA
TTTT

Answer: C

DNA technology has many medical applications. Which of the following is not done routinely at present?

DNA technology has many medical applications. Which of the following is not done routinely at present? 

A) production of hormones for treating diabetes and dwarfism
B) production of viral proteins for vaccines
C) introduction of genetically engineered genes into human gametes
D) prenatal identification of genetic disease genes
E) genetic testing for carriers of harmful alleles

Answer: C

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of 

A) post-transcriptional processing.
B) electroporation.
C) post-translational processing.
D) nucleic acid hybridization.
E) restriction fragment ligation.

Answer: A