Energy released by the electron transport chain is used to pump H+ ions into which location? Naim 12:06 AP Biology Chapter 9 Energy released by the electron transport chain is used to pump H+ ions into which location? A. Cytosol B. Mitochondrial outer membrane C. Mitochondrial inner membrane D. Mitochondrial intermembrane space E. Mitochondrial matrix Answer: D Learn More : Share this Share on FacebookTweet on TwitterPlus on Google+