Which uses reverse transcriptase to make cDNA followed by amplification?
A) Southern blotting
B) Northern blotting
C) Western blotting
D) Eastern blotting
E) RT-PCR
Answer: E
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AP Biology Chapter 20
- In recombinant DNA methods, the term vector can refer to
- Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme?
- DNA technology has many medical applications. Which of the following is not done routinely at present?
- Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of
- A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird). The researcher would like to compare a specific region of the DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of dodo DNA available for testing?
- Plants are more readily manipulated by genetic engineering than are animals because
- Which of the following would not be true of cDNA produced using human brain tissue as the starting material?
- Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
- As genetic technology makes testing for a wide variety of genotypes possible, which of the following is likely to be an increasingly troublesome issue?
- One possible use of transgenic plants is in the production of human proteins, such as vaccines. Which of the following is a possible hindrance that must be overcome?
- Which of the following is one of the technical reasons why gene therapy is problematic?
- One successful form of gene therapy has involved delivery of an allele for the enzyme adenosine deaminase (ADA) to bone marrow cells of a child with SCID, and delivery of these engineered cells back to the bone marrow of the affected child. What is one major reason for the success of this procedure as opposed to many other efforts at gene therapy?
- Scientists developed a set of guidelines to address the safety of DNA technology. Which of the following is one of the adopted safety measures?
- Genetically engineered plants
- Genetic engineering is being used by the pharmaceutical industry. Which of the following is not currently one of the uses?
- Gene therapy
- Which of the following are probably siblings?
- Which of the following statements is most likely true?
- Which of the following statements is consistent with the results?
- A researcher is using adult stem cells and comparing them to other adult cells from the same tissue. Which of the following is a likely finding?
- Which of the following is true of embryonic stem cells but not of adult stem cells?
- Reproductive cloning of human embryos is generally considered unethical. However, on the subject of therapeutic cloning there is a wider divergence of opinion. Which of the following is a likely explanation?
- Which of the following problems with animal cloning might result in premature death of the clones?
- In 1997, Dolly the sheep was cloned. Which of the following processes was used?
- Which of the following is most closely identical to the formation of twins?